Induction, detection, formation, and resuscitation of viable but non‐culturable state microorganisms
نویسندگان
چکیده
منابع مشابه
Formation and Resuscitation of Viable but Nonculturable Salmonella typhi
Salmonella typhi is a pathogen that causes the human disease of typhoid fever. The aim of this study was to investigate the viable but nonculturable (VBNC) state of S. typhi. Some samples were stimulated at 4°C or -20°C, while others were induced by different concentrations of CuSO4. Total cell counts remained constant throughout several days by acridine orange direct counting; however, plate c...
متن کاملThe viable but nonculturable state and cellular resuscitation
Aquatic microbial ecologists have long recognized that portions of bacterial populations seem to disappear from natural water bodies during certain times of the year, only to reappear at other times. In the case of V. vulnificus, numerous studies have documented the difficulty researchers experience in culturing this bacterium during cold months, presumably due to “die-off” of the population. T...
متن کاملResuscitation of Vibrio vulnificus from the Viable but Nonculturable State.
Like many other gram-negative bacteria, the human pathogen Vibrio vulnificus is induced into a viable but nonculturable (VBNC) state by incubation at low temperatures. The ability of any bacterium to resuscitate from this dormant state would appear to be essential if the VBNC state is truly a survival strategy. The question as to whether the culturable cells which appear following removal of th...
متن کاملThe viable but nonculturable state in bacteria.
It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow. This mini-review defines what the "viable but nonculturable" (VBNC) state is, and illustrates the methods that ca...
متن کاملInduction and resuscitation of viable but nonculturable Salmonella enterica serovar typhimurium DT104.
Salmonella enterica serovar Typhimurium DT104 11601 was tested for its ability to maintain viability in minimal, chemically defined solutions. Periodic monitoring of growth and survival in microcosms of different ion concentrations, maintained at various temperatures, showed a gradual decline in culturable organisms ( approximately 235 days) at 5 degrees C. Organisms maintained at a higher temp...
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ژورنال
عنوان ژورنال: Comprehensive Reviews in Food Science and Food Safety
سال: 2019
ISSN: 1541-4337,1541-4337
DOI: 10.1111/1541-4337.12513